chemistry department instrumentation
The Huntington University Chemistry
Department’s collection of instrumentation was enhanced when the Science
Hall was built in 2002. Our instruments have been carefully chosen to
be student-friendly and to represent a range of analyses encountered in
both industry and research. Chemistry majors at Huntington have the
advantage of being mentored in the operation and maintenance of these
Varian Gemini 200 MHz nuclear magnetic resonance (NMR) spectrometer is a
research-grade instrument that allows students to solve structures of
molecules. Understanding how the instrument functions also allows
students to better comprehend the related technique of magnetic
resonance imaging (MRI) in medicine.
||The Shimadzu QP5000 gas chromatograph –
mass spectrometer (GC-MS) is an industrial-type instrument which allows
students to separate, quantify, and identify components of volatile
mixtures with the aid of electronic databases.
||The two Fourier transform infrared
spectrophotometers (FTIRs) (Bruker Vector 33 and Mattson Galaxy 2020)
allow students to identify molecules based on “fingerprint” spectra that
they generate when they absorb infrared radiation. Accessories allow
students to use the technique to identify materials in many different
forms including powders, gases, liquids, fabrics, and adhesives.
||The three UV-Visible spectrophotometers
(Perkin Elmer Lambda 35, Lambda 3, and Lambda 1) allow students to
quantify the concentrations of colored substances or substances that
absorb UV radiation.
two fluorimeters (Ocean Optics spectrofluorimeter and Turner filter
fluorimeter) use the very sensitive technique of fluorescence
spectroscopy to detect small quantities of fluorescent materials such as
chlorophyll or fluorescent biological molecules. Shown in the photo is one of our
smallest-sized instruments, an Ocean Optics fiber-optic fluorimeter.
The SRI 8610C gas chromatograph (GC)
allows students to separate components of mixtures with low boiling
points. This modern workhorse instrument allows students to practice
their sample injection techniques and “tinker” with the instrument.
The Pine WaveNow potentiostat allows
students to measure the concentrations and oxidation states of metals
and other substances using electrodes immersed in solution, through
techniques such as cyclic voltammetry (CV).
The Varian 5000 high performance liquid
chromatograph (HPLC) allows students to separate and quantify components
of non-volatile mixtures such as food ingredients or pharmaceuticals.
The laser polarimeter, constructed
in-house, allows students to observe and quantify the rotation of light
caused by optically active substances.
||The Parr oxygen combustion and solution
calorimeters (Models 1341 and 1451) allow students to quantify the heat
released or absorbed in various reactions, using a technique used in
industry to measure Calories in food items.
The Vernier LabQuest interfaces are used
in the introductory labs to allow students to collect data
electronically in order to measure and graph quantities such as
temperature, pH, and voltage.
The Wavefunction SpartanPro software
allows students to use the laboratory laptop computers to calculate and
visualize properties of individual molecules such as charge density,
bond lengths, geometries, and vibrational frequencies.